Biotin-PEG-Azide

The Biotin-PEGn-CH₂CH₂N₃ series, exemplified by Biotin-PEG4-CH₂CH₂N₃, is a heterobifunctional reagent designed for bioorthogonal click chemistry applications. It enables efficient labeling and conjugation of biomolecules through copper-catalyzed (CuAAC) or strain-promoted (SPAAC) azide-alkyne cycloaddition with alkyne-modified targets. The extended PEG spacer significantly reduces steric hindrance and enhances aqueous solubility, while the terminal biotin moiety facilitates robust capture or detection via high-affinity streptavidin-biotin interaction. This combination makes the series particularly valuable for diverse applications including biomolecular imaging, affinity purification, and diagnostic assay development.