Maleimide-(CH2)n-COOH

Maleimide-(CH₂)n-COOH is a heterobifunctional reagent designed for sequential thiol-specific conjugation via maleimide chemistry followed by carboxyl-mediated amidation with amines. Its key advantages include tunable spacer length (n) to optimize distance and flexibility, orthogonal reactivity for controlled biomolecular assembly, and stable amide bond formation, though the maleimide group is sensitive to hydrolysis and may require anhydrous handling.