Bis-Maleimide-Amide PEG

Mal-NH-PEGn-NH-Mal is a heterotrifunctional reagent engineered for multi-step bioconjugation, enabling dual maleimide-mediated thiol coupling and amine-based modifications via the central amino group. Its key advantages include orthogonal reactivity for complex biomolecular assemblies, tunable spacer length (n), and enhanced solubility, though the maleimide groups require protection against hydrolysis and the amine may need capping during thiol-directed reactions to prevent cross-reactivity.