Maleimide-(CH2)5-CONH-CH2CH2NH2
Maleimide-(CHâ‚‚)â‚…-CONH-CHâ‚‚CHâ‚‚NHâ‚‚ is a heterobifunctional reagent
designed for sequential thiol-specific conjugation via maleimide chemistry followed by amine-mediated modifications (e.g., amidation) through the terminal primary amine. Its key advantages include an extended hydrophobic spacer for reduced steric hindrance, orthogonal reactivity for flexible biomolecular assembly, and enhanced stability of the amide-linked chain, though the maleimide group requires protection against hydrolysis and the amine may need capping during thiol-directed reactions.
