Maleimide-(CH2)n-CO-NHNH2.TFA

Maleimide-(CH₂)n-CO-NHNH₂·TFA is a heterobifunctional reagent designed for sequential thiol-specific conjugation via maleimide chemistry followed by hydrazone formation with carbonyl groups (e.g., in oxidized antibodies or glycoproteins). Its key advantages include orthogonal reactivity for site-specific labeling, tunable spacer length (n) to reduce steric interference, and enhanced solubility due to the TFA salt form, though the maleimide group requires protection against hydrolysis and the hydrazide may exhibit limited stability in acidic environments.